Vitamin B12 derivatives catalyze a wide variety of organic transformations, but B12-dependent enzymes are underutilized in biocatalysis relative to other metalloenzymes. In this study, we engineered a variant of your transcription aspect CarH, called CarH*, that catalyzes styrene C-H alkylation with improved yield and selectivity relative to B12 itself. Though the native function of CarH includes transcription regulation by means of AdoCbl Co(III)-carbon bond cleavage and 尾-hydride elimination to create 4鈥?5鈥?didehydroadenosine, CarH*-catalyzed styrene alkylation proceeds by way of non-native oxidative addition and olefin addition coupled having a native-like 尾-hydride elimination. Mechanistic research on this reaction echo findings from earlier research on AdoCbl homolysis under robust cage situations to recommend that CarH* can enable non-native radical chemistry with improved selectivity relative to B12 itself. These findings lay the groundwork for the improvement of B12-dependent enzymes as catalysts to get a wide range of non-native transformations. 3-Aminopicolinaldehyde Price 2-Fluoro-4-methoxynicotinic acid web PMID:23600560