DNA amplification detection is {typically|usually|normally|generally|commonly|ordinarily} a lengthy and sophisticated {procedure|process} involving high-end instrumentation. Electrochemical detection has {recently|lately|not too long ago} opened a {relatively|fairly|comparatively|reasonably|somewhat} low {budget|spending budget|price range}, a {straightforward|simple} and speedy {method|technique|approach|strategy|system|process} for detection {with the|using the|with all the|together with the} {aid|help} of an electrochemically active redox probe. {However|Nevertheless|Nonetheless|Even so|On the other hand|Having said that}, electrochemical sensors of nucleic acid amplification characteristically employ a tri-electrode geometry involving {standard|regular|normal|common|typical} reference electrode {usually|generally|typically|normally|commonly|ordinarily} {made|produced|created} up of a noble metal that adds up {the cost|the price} of detection. This {work|function|perform|operate} proposes a sensitive and {rapid|fast|speedy} {approach|method|strategy} for detecting the endpoint of nucleic acid amplification test {using|utilizing|making use of|employing|working with|applying} a novel bi-electrode sensing geometry. The sensing layer {of the|from the|in the|on the|with the|of your} electrode was comprised of a transition metal oxide to tune its electronic state for regulating its electrochemical response. The fabricated device was then {used|utilized|employed|utilised|applied|made use of} to detect dengue virus sequence DNA ({using|utilizing|making use of|employing|working with|applying} rolling circle amplification) and Staphylococcus aureus genomic DNA ({using|utilizing|making use of|employing|working with|applying} polymerase chain reaction) {with the|using the|with all the|together with the} limit of detection {in the|within the|inside the} order of 103 target DNA copies. Buy1207625-15-7 Methyltetrazine-Amine web PMID:24293312
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