The activity orchestration of an unprecedented cell-free enzyme {system|method|program|technique} with self-sufficient cofactor recycling enables the step-wise transformation of aliphatic diols into w-hydroxy acids {at the|in the} expense of molecular oxygen as electron acceptor. The efficiency {of the|from the|in the|on the|with the|of your} biosynthetic route was maximized when two compatible alcohol dehydrogenases {were|had been|have been} {selected|chosen} as specialist biocatalysts for {each|every|each and every|every single} {one of the|among the|one of many|one of several|among the list of|on the list of} oxidative {steps|actions|measures|methods} {required|needed|necessary|essential|expected} for the oxidative lactonization of diols. The cell-free {system|method|program|technique} reached {up to|as much as} 100% conversion {using|utilizing|making use of|employing|working with|applying} {100|one hundred} mM of linear C5 diols, and performed the dessymetrization of prochiral branched diols {into the|in to the} corresponding w-hydroxy acids with an exquisite enantioselectivity (ee > 99%). Green metrics demostrate a superior sustanability of this {system|method|program|technique} {compared to|in comparison to|in comparison with|when compared with} {traditional|conventional|standard|classic|regular} metal catalysts {and even|as well as|and also|and in some cases} to {whole|entire|complete} cells for the synthesis of 5-hydroxy petanoic acid. {Finally|Lastly|Ultimately}, the cell-free {system|method|program|technique} was assembled into a consortium of heterogeneous biocatalysts that {allowed|permitted} the enzyme reutilization. This cascade illustrates the {potential|possible|prospective} of systems biocatalysis to access new heterofunctional molecules {such as|like|including|for example|for instance|which include} w-hydroxy acids. 2096419-56-4 Chemscene 36294-24-3 Price PMID:23907051
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