We present a microfluidic device for selective separation and extraction of molecules {based|primarily based} on their diffusivity. The separation relies on electroosmotically-driven bidirectional flows in which {high|higher} diffusivity species {experience|encounter|expertise|knowledge|practical experience} a net-zero velocity, and {lower|reduce|reduced|decrease} diffusivity species are advected to a collection reservoir. The device can operate {continuously|constantly} and is {suitable|appropriate} for processing low sample volumes. {Using|Utilizing|Making use of|Employing|Working with|Applying} {several|a number of|numerous|many|various|quite a few} model systems, we showed that the extraction efficiency {of the|from the|in the|on the|with the|of your} {system|method|program|technique} is maintained at {more than|greater than} 90% {over|more than} tens of minutes, {with a|having a|using a} purity of {more than|greater than} 99%. We demonstrate the applicability {of the|from the|in the|on the|with the|of your} device {to the|towards the|for the} extraction of genomic DNA from {short|brief|quick} DNA fragments. 1000575-20-1 Purity 1-Hydroxyhept-6-yn-3-one Formula PMID:23460641
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