DNA-based nanostructures (DNs) are advantageous for the {design|style|design and style} of functional {materials|supplies|components} for biology and medicine {due to|because of|as a result of|on account of|resulting from|as a consequence of} the nanoscale {control|manage|handle} {provided|supplied|offered} by their predictable self-assembly. {However|Nevertheless|Nonetheless|Even so|On the other hand|Having said that}, {the use of|the usage of} DNs in vivo has been {limited|restricted} {due to|because of|as a result of|on account of|resulting from|as a consequence of} structural instability in biofluids. {As the|Because the} stability of {a particular|a specific|a certain} DN sets the scope of its {potential|possible|prospective} biological applications, {efficient|effective} {methods|techniques|strategies|approaches|procedures|solutions} to characterize stability are {required|needed|necessary|essential|expected}. {Here|Right here}, we apply size exclusion chromatography (SEC) to study the stability of a tetrahedron DNA nanostructure (TDN) and demonstrate the analytical capabilities of our {method|technique|approach|strategy|system|process} in characterizing degradation by enzymes {and a|along with a|as well as a|plus a|and also a|in addition to a} diluted human serum matrix. We show that SEC {analysis|evaluation} can reliably assay TDN degradation by a nuclease {through|via|by means of|by way of} direct injection and peak integration. {Furthermore|Moreover|In addition|Additionally}, {data|information} {analysis|evaluation} {using|utilizing|making use of|employing|working with|applying} a ratio chromatogram {technique|method|approach|strategy} enables TDN peak deconvolution {from the|in the} matrix of serum proteins. {Using|Utilizing|Making use of|Employing|Working with|Applying} our {method|technique|approach|strategy|system|process}, we {found|discovered|identified|located} that TDNs exhibit half-lives of 23.9 hours and {10|ten}.1 hours in 20% and 50% diluted human serum, respectively, {which is|that is|which can be} {consistent|constant} with reported stability {studies|research} in 10% fetal bovine serum. We anticipate that this {method|technique|approach|strategy|system|process} {could be|might be|could possibly be|may be|may very well be} broadly applicable to characterize {a variety of|a number of|many different|various|a range of|several different} DNs and serve as an {efficient|effective} {technique|method|approach|strategy} toward {analysis|evaluation} {of the|from the|in the|on the|with the|of your} stability of new DN {designs|styles} in {complex|complicated} biological matrixes. 16200-85-4 web Methyl 4-bromo-1H-indole-7-carboxylate In stock PMID:24463635
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