Liquid chromatography coupled to mass spectrometry {is a|is really a|is actually a|can be a|is often a|is usually a} {key|important|crucial|essential} metabolomics {technology|technologies}. Reversed-phase liquid chromatog-raphy (RPLC) {is very|is extremely|is quite} {widely|extensively|broadly} {used|utilized|employed|utilised|applied|made use of} as a separation step, possessing {excellent|superb|outstanding|exceptional|great|fantastic} {characteristics|traits|qualities} with respect to reproducibility and reliability, but {typically|usually|normally|generally|commonly|ordinarily} has poor retention of {highly|extremely|very|hugely} polar metabolites. {Here|Right here}, we evaluated the {combination|mixture} of two alter-native {methods|techniques|strategies|approaches|procedures|solutions} for {improving|enhancing} retention of polar metabolites {based on|according to|depending on|determined by} 6-aminoquinoloyl-N-hydroxysuccinidimyl carbamate derivatization for amine groups, and ion-pairing chromatography (IPC) {using|utilizing|making use of|employing|working with|applying} tributylamine as an ion-pairing agent to retain acids. We compared {both|each} {of these|of those} {methods|techniques|strategies|approaches|procedures|solutions} to RPLC {and also|as well as} to {each|every|each and every|every single} other, for targeted {analysis|evaluation} {using|utilizing|making use of|employing|working with|applying} a triple-quadrupole mass spectrometer, applied to a library of ca. 500 polar metabolites. IPC and derivatization {were|had been|have been} complementary {in terms of|when it comes to|with regards to} their coverage: combined, they {improved|enhanced} the proportion of metabolites with {good|great|excellent|very good|fantastic|superior} retention to 91%, {compared to|in comparison to|in comparison with|when compared with} just 39% for RPLC alone. We detected 132 metabolites for {real|genuine|actual|true} biological samples (liver extracts) with {good|great|excellent|very good|fantastic|superior} reproducibility ({based on|according to|depending on|determined by} coefficients of variation in pooled biological {quality|high quality|top quality|good quality|excellent|high-quality} {control|manage|handle} samples). {Finally|Lastly|Ultimately}, we tested the {combination|mixture} of {methods|techniques|strategies|approaches|procedures|solutions} with real-world samples by analyzing a set of liver extracts from aged male and female mice that had been treated {with the|using the|with all the|together with the} poly-phenol compound ampelopsin. {Furthermore|Moreover|In addition|Additionally}, we also compared {the results|the outcomes} {of these|of those} LC-MS {methods|techniques|strategies|approaches|procedures|solutions} to 1H NMR spectrosco-py as an orthogonal {method|technique|approach|strategy|system|process} (also termed statistical heterospectroscopy (SHY)), and {found|discovered|identified|located} a {strong|powerful|robust|sturdy} correlation {between|in between|among|amongst|involving} {the results|the outcomes} {of these|of those} {different|various|distinct|diverse|unique|distinctive} analytical approaches. By these {means|indicates|implies|signifies|suggests}, {not only|not just|not merely|not simply} {were|had been|have been} {a number of|numerous|several|quite a few|a variety of|many} {significantly|considerably|substantially|drastically} changed metabolites detected, {but also|but additionally|but in addition} it {could be|might be|could possibly be|may be|may very well be} shown that there was a clear interaction {between|in between|among|amongst|involving} ampelopsin {treatment|therapy|remedy} and sex, in that the di-rection of metabolite {change|alter|modify|adjust|transform} was opposite for males and females. 935845-20-8 Chemscene 201732-49-2 site PMID:24318587
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