{In this|Within this} {work|function|perform|operate}, we present a {simple|easy|straightforward|basic|uncomplicated|very simple}, {straightforward|simple}, and robust {method|technique|approach|strategy|system|process} for spontaneously digitizing samples into an array of dis-crete volumes. The {method|technique|approach|strategy|system|process} is {based on|according to|depending on|determined by} an oil-triggered, template-confined dewetting phenomenon. To {realize|understand|recognize|comprehend} the dewetting-induced sample digitization, an aqueous sample is firstly infused into a networked microwell array (NMA) {through|via|by means of|by way of} a predegassing-based self-pumping mechanism, {and then|and after that|after which|then} an immiscible oil phase is applied {over|more than} the surface of NMA chip to induce the templated dewetting. {Due to|Because of|As a result of|On account of|Resulting from|As a consequence of} a periodic interfacial tension heterogeneity, such dewetting ruptures the sample {at the|in the} thinnest {parts|components} (i.e., con-nection channels) and spontaneously splits the sample into droplets in {individual|person} microwells. {Without|With out|Without having|With no|Devoid of|Without the need of} requiring any {complex|complicated} pump-ing or valving systems, this {method|technique|approach|strategy|system|process} can discretize a sample into tens of {thousands of|a large number of|a huge number of} addressable droplets {in a|inside a|within a} matter of minutes with {nearly|almost|practically} 98% usage. To demonstrate the utility and universality of this self-digitization {method|technique|approach|strategy|system|process}, we exploited it to discretize samples into 40,233 wells for digital PCR assay, digital quantification of bacteria, and self-assembly of spherical colloidal photonic crystals. We {believe|think} this facile {technique|method|approach|strategy} {will be|will probably be|will likely be|is going to be|might be|are going to be} {useful|helpful|beneficial|valuable} {in a|inside a|within a} broad {range of|selection of|array of} applications {where|exactly where} partitioning of samples into {a large|a sizable|a big} {number of|quantity of|variety of} {small|little|tiny|modest|smaller|compact} {individual|person} volumes is {required|needed|necessary|essential|expected}. Boc-NH-PEG3 Chemical name Price of 2-Chloro-1H-indole PMID:24140575
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