We introduce a {strategy|technique|method|approach|tactic} for the fluorogenic and genetic targeting of a calcium sensor by combining a protein fluorogen {with the|using the|with all the|together with the} BAPTA sensing group. The resulting dual-input probe acts like a fluorescent AND logic gate {with a|having a|using a} Ca2+-sensitive red emission {that is|that’s|which is|that is certainly|that is definitely|that may be} activated only upon reaction {with the|using the|with all the|together with the} protein self-labeling tag HaloTag {with a|having a|using a} 18-fold intensity enhancement. The fluorogenic targeting {of the|from the|in the|on the|with the|of your} calcium probe was evidenced by the selective wash-free imaging of calcium {in the|within the|inside the} nucleus of Hela cells expressing a nuclear HaloTag protein. 1801273-41-5 Purity 1089706-28-4 structure PMID:23833812
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