Selenoprotein F (SelF) {is an|is definitely an} endoplasmic reticulum-residing eukaryotic protein that {contains|consists of|includes} a selenocysteine (Sec) residue. It has been {suggested|recommended} {to be|to become} involved {in a|inside a|within a} {number of|quantity of|variety of} physiological processes by acting as a thiol-disulfide oxidoreductase, {but the|however the} {exact|precise} {role|function|part} has remained unclear {due to|because of|as a result of|on account of|resulting from|as a consequence of} the lack of a {reliable|dependable|trustworthy|reputable|trusted} production {method|technique|approach|strategy|system|process}. We document herein a robust synthesis {of the|from the|in the|on the|with the|of your} human SelF {through|via|by means of|by way of} a three-segment two-ligation semisynthesis {strategy|technique|method|approach|tactic}. Highlighted {in this|within this} synthetic route {are the|would be the|will be the} use of a mild desulfurization {process|procedure|method|approach|course of action} to {protect|shield|safeguard|defend|guard} the side-chain {of the|from the|in the|on the|with the|of your} Sec residue from {being|becoming|getting} {affected|impacted} {and the|and also the|as well as the|along with the|plus the} simultaneous removal of acetamidomethyl and p-methoxybenzyl protection groups by PdCl2, {thus|therefore|hence|as a result} facilitating the synthesis of multi-milligram of homogenous SelF. The reduction {potential|possible|prospective} of SelF was determined {and the|and also the|as well as the|along with the|plus the} thiol-disulfide oxidoreductase activity was {further|additional} supported by its {ability to|capability to} catalyze the reduction and isomerization of disulfide bonds. 77545-45-0 Price N,N’-Diisopropylcarbodiimide(DIC) Chemical name PMID:23514335
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