Doi.org/10.1371/journal.pone.0178375 May possibly 25,14 /Effects of TH588 in NETsQGP1) just after 96 h of incubation with TH588 (five M or ten M) alone or in combination with 5FU (five M) or everolimus (ten nM). (TIF) S26 Fig. Uncropped Western blot of Actin and PCNA expression in combinational therapy. Expression of Actin and cleaved PCNA in neuroendocrine cell lines (BON1, H727 and QGP1) after 96 h of incubation with TH588 (5 M or ten M) alone or in mixture with 5FU (five M) or everolimus (ten nM). (TIF) S27 Fig. Uncropped Western blot of Actin and PCNA expression in combinational therapy. Expression of Actin and cleaved PCNA in neuroendocrine cell lines (BON1, H727 and QGP1) just after 96 h of incubation with TH588 (five M or ten M) alone or in mixture with 5FU (five M) or everolimus (10 nM). (TIF) S28 Fig. Uncropped Western blot of Chk1, CDK1, Bcl2 and p21 expression in combinational remedy. Expression of Chk1, CDK1, Bcl2 and p21 in neuroendocrine cell lines (BON1, H727 and QGP1) after 96 h of incubation with TH588 (5 M or ten M) alone or in combination with 5FU (five M) or everolimus (10 nM). (TIF) S29 Fig. Uncropped Western blot of Chk1, CDK1, Bcl2 and p21 expression in combinational treatment.Fmoc-8-Aoc-OH web Expression of Chk1, CDK1, Bcl2 and p21 in neuroendocrine cell lines (BON1, H727 and QGP1) after 96 h of incubation with TH588 (five M or 10 M) alone or in combination with 5FU (five M) or everolimus (10 nM).2092067-90-6 Purity (TIF) S30 Fig.PMID:23829314 Uncropped Western blot of Chk1, CDK1, Bcl2 and p21 expression in combinational therapy. Expression of Chk1, CDK1, Bcl2 and p21 in neuroendocrine cell lines (BON1, H727 and QGP1) just after 96 h of incubation with TH588 (5 M or 10 M) alone or in mixture with 5FU (5 M) or everolimus (ten nM). (TIF) S31 Fig. Uncropped Western blot of Chk2, CDK4 and CDK6 expression in combinational therapy. Expression of Chk2, CDK4 and CDK6 in neuroendocrine cell lines (BON1, H727 and QGP1) following 96 h of incubation with TH588 (five M or 10 M) alone or in combination with 5FU (5 M) or everolimus (ten nM). (TIF) S32 Fig. Uncropped Western blot of Chk2, CDK4 and CDK6 expression in combinational remedy. Expression of Chk2, CDK4 and CDK6 in neuroendocrine cell lines (BON1, H727 and QGP1) soon after 96 h of incubation with TH588 (five M or 10 M) alone or in mixture with 5FU (five M) or everolimus (10 nM). (TIF) S33 Fig. Uncropped Western blot of Chk2, CDK4 and CDK6 expression in combinational remedy. Expression of Chk2, CDK4 and CDK6 in neuroendocrine cell lines (BON1, H727 and QGP1) just after 96 h of incubation with TH588 (five M or 10 M) alone or in mixture with 5FU (five M) or everolimus (ten nM). (TIF)PLOS One | https://doi.org/10.1371/journal.pone.0178375 May 25,15 /Effects of TH588 in NETsS34 Fig. Uncropped Western blot of MTH1 expression in combinational therapy. Expression of MTH1 in neuroendocrine cell lines (BON1, H727 and QGP1) following 96 h of incubation with TH588 (5 M or ten M) alone or in mixture with 5FU (five M) or everolimus (ten nM). (TIF) S35 Fig. Uncropped Western blot of MTH1 expression in combinational therapy. Expression of MTH1 in neuroendocrine cell lines (BON1, H727 and QGP1) immediately after 96 h of incubation with TH588 (five M or 10 M) alone or in combination with 5FU (five M) or everolimus (10 nM). (TIF) S36 Fig. Uncropped Western blot of MTH1 expression in combinational remedy. Expression of MTH1 in neuroendocrine cell lines (BON1, H727 and QGP1) soon after 96 h of incubation with TH588 (5 M or ten M) alone or in combination with 5FU (five M) or everolimus (ten nM). (TIF) S37 Fig. Un.