The vesicular anion transport activity assay, which {uses|utilizes|makes use of} 8-hydroxypyrene-1,{3|three},6-trisulfonic acid to monitor the internal pH {of the|from the|in the|on the|with the|of your} vesicles (the HPTS assay), {is a|is really a|is actually a|can be a|is often a|is usually a} {widely|extensively|broadly} {used|utilized|employed|utilised|applied|made use of} {technique|method|approach|strategy} for analysing the activity of anionophore facilitated transport across a phospholipid membrane. This {methods|techniques|strategies|approaches|procedures|solutions} paper describes the stepwise {technique|method|approach|strategy} to conduct this transport assay, detailing {both|each} the perks and pitfalls of {using|utilizing|making use of|employing|working with|applying} this {method|technique|approach|strategy|system|process} to {determine|figure out|decide|establish|ascertain|identify} the activity of an anionophore {and the|and also the|as well as the|along with the|plus the} transport mechanism. Buy751470-47-0 1250997-56-8 Chemscene PMID:23695992
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