{In this|Within this} publication, we {developed|created} the {high|higher} throughput screening implementation of freeze-float {selection|choice} platform {system|method|program|technique} we established {in the|within the|inside the} {previous|prior|earlier|preceding} publication. The {goal|objective|aim|purpose|target} of this publication {is to|would be to|is always to|is usually to|will be to|should be to} expand the {system|method|program|technique} to {higher|greater|larger} throughput and accuracy. {In the|Within the|Inside the} following sections, we describe the {steps|actions|measures|methods} for automated droplets generations, adaptation of freeze-float {selection|choice} {using|utilizing|making use of|employing|working with|applying} controlled and uniform temperature cooling {system|method|program|technique}, and semi-automated droplets detection {program|plan|system}. We aimed {to improve|to enhance} previously published {system|method|program|technique} to add functional {advantages|benefits|positive aspects}, {such as|like|including|for example|for instance|which include}; a) {increased|elevated|improved|enhanced} efficiency {of the|from the|in the|on the|with the|of your} screening with fewer manipulation {steps|actions|measures|methods}; b) {increased|elevated|improved|enhanced} accuracy of measurement {due to|because of|as a result of|on account of|resulting from|as a consequence of} the {increased|elevated|improved|enhanced} sample size; c) {increased|elevated|improved|enhanced} uniformity of temperature distribution by incorporating the controlled-rate freezer. {In the|Within the|Inside the} following sections, we describe the {steps|actions|measures|methods} for automated droplets generations, freeze-float {system|method|program|technique} adaptations {using|utilizing|making use of|employing|working with|applying} controlled and uniform temperature cooling {system|method|program|technique}, and semi-automated droplets detection {program|plan|system}. 3-Penten-2-one Data Sheet 2-(2-Bromoethyl)oxirane Formula PMID:23291014
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