Prenylation plays {an important|an essential|a crucial|a vital} {role|function|part} in diversifying structure and function of secondary metabolites. {Although|Even though|Though|Despite the fact that|While} {several|a number of|numerous|many|various|quite a few} cyanobactin prenyltransferases {have been|happen to be|have already been} characterized, their modes of action are {mainly|primarily|mostly} {limited|restricted} {to the|towards the|for the} modification of electron-rich hetero atoms. {Here|Right here} we report a {unique|distinctive|special|exclusive|exceptional|one of a kind} prenyltransferase originating from Limnothrix sp. CACIAM 69d, {referred to as|known as} LimF, which catalyzes an unprecedented His-C-geranylation. Interestingly, LimF executes the geranylation on {not only|not just|not merely|not simply} its native peptide substrate {but also|but additionally|but in addition} a wide {range of|selection of|array of} exotic peptides, {including|such as|which includes|like} thioether-closed macrocycles. {We have|We’ve|We’ve got} also serendipitously uncovered an {ability|capability|capacity|potential} of Tyr-O-geranylation {as the|because the} secondary function of LimF, indicating {it is|it’s|it really is|it truly is|it can be|it is actually} an {unusual|uncommon} bifunctional prenyltransferase. Crystallographic {analysis|evaluation} of LimF complexed {with a|having a|using a} pentapeptide substrate {and a|along with a|as well as a|plus a|and also a|in addition to a} prenyl donor analog {provides|offers|gives|supplies|delivers} structural basis for its {unique|distinctive|special|exclusive|exceptional|one of a kind} His recognition and its bifunctionality. Lastly, we show the LimF’s prenylation {ability|capability|capacity|potential} on {various|numerous|different|a variety of|several|many} bioactive molecules containing an imidazole group, highlighting its {potential|possible|prospective} as a versatile biocatalyst for site-specific geranylation. 4-Chloro-5-methoxypyridin-2-amine Purity 6-Formylnicotinonitrile site PMID:35954127
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