{Despite|Regardless of|In spite of} the {enormous|huge|massive} {importance|significance|value} of insoluble proteins in biological processes, their structural investigation remains a {challenging|difficult} {task|job|activity|process}. {Development|Improvement} of artificial enzymatic catalysts would {greatly|significantly|tremendously|drastically|considerably} facilitate elucidation of their structure as {currently|presently|at present|at the moment} {used|utilized|employed|utilised|applied|made use of} enzymes in proteomics largely {lose|shed|drop} activity {in the|within the|inside the} presence of surfactants, {which are|that are} {necessary to|essential to} solubilize insoluble proteins. {In this|Within this} study the hydrolysis of a {fully|totally|completely} insoluble protein by a polyoxometalate {complex|complicated} as an artificial protease in surfactant {solutions|options} is reported for {the first|the very first|the initial} time. The hydrolysis of zein as a model protein was investigated {in the|within the|inside the} presence of Zr(IV)-substituted Keggin-type polyoxometalate (POM), (Et2NH2)10[Zr(α-PW11O39)2], and {different|various|distinct|diverse|unique|distinctive} concentrations {of the|from the|in the|on the|with the|of your} anionic surfactant sodium dodecyl sulfate (SDS). The selective hydrolysis {of the|from the|in the|on the|with the|of your} protein upon incubation {with the|using the|with all the|together with the} catalyst was observed, {and the|and also the|as well as the|along with the|plus the} {results|outcomes|final results|benefits} indicate that hydrolytic selectivity and activity {of the|from the|in the|on the|with the|of your} POM catalysts strongly {depends on|depends upon|is determined by|is dependent upon|will depend on} the concentration of surfactant. The molecular interactions {between|in between|among|amongst|involving} the POM catalyst and zein {in the|within the|inside the} presence of SDS {were|had been|have been} explored {using|utilizing|making use of|employing|working with|applying} a {combination|mixture} of spectroscopic {techniques|methods|strategies|tactics|approaches|procedures} which indicated competitive binding {between|in between|among|amongst|involving} POM and SDS towards the protein. The formation of micellar superstructures in tertiary POM/surfactant/protein {solutions|options} has been confirmed by electrical conductivity and Dynamic Light Scattering. 2,6-Dibromopyridin-4-amine Chemical name Price of Palladium (trifluoroacetate) PMID:24631563
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