{Despite|Regardless of|In spite of} the prevalence of ortho- and pyrophosphate in biochemistry, operationally {simple|easy|straightforward|basic|uncomplicated|very simple} and versatile high-throughput methodologies for their quantification are lacking. We herein introduce PUB, a module for phosphate detection by continuous UV-spectroscopic monitoring of 5-bromouridine phosphorolysis. The PUB module employs cheaply {available|accessible|obtainable|offered|readily available|out there}, bench-stable reagents {and can|and may} be employed for continuous and discontinuous reaction monitoring in biochemical assays to detect (pyro-)phosphate concentrations spanning {almost|nearly|virtually|practically|just about|pretty much} {four|4} orders of magnitude, as demonstrated with representative use-cases. Estrone site 2-Aminoacetamide Chemscene PMID:32695810
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